KMID : 0861120130170030165
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Korean Journal of Oriental Preventive Medicine 2013 Volume.17 No. 3 p.165 ~ p.176
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Simultaneous Determination of Gumiganghwal-tang and Its Anti-atherosclerotic Effect
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Kim Ohn-Soon
Seo Chang-Seob Shin Hyeun-Kyoo
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Abstract
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Objective : The purpose of this study was to establish the simultaneous analysis for six compounds in Gumiganghwal-tang (GMGHT, Jiuweiqianghuo-tang) and to investigate the anti-atherosclerotic effects of GMGHT in vitro
Methods : The column for separation of six compounds was used Luna C18 column and maintained at 40¡É. The mobile phase for gradient elution consisted of two solvent systems, 1.0% acetic acid in water and 1.0% acetic acid in acetonitrile. The analysis was carried out at a flow rate of 1.0 mL/min with pothodiode array (PDA) detection at 254, 280, and 320 nm. The injection volume was 10 ¥ìL. The antioxidant activities of GMGHT were evaluated by measuring free radical scavenging activities on 2,2'-Azinobis-3-ethyl-benzothiazoline-6-sulfonic acid (ABTS) and 1-1-diphenyl-2-picrylhydrazyl (DPPH). The inhibitory effects on low-density lipoprotein (LDL) oxidation were evaluated by the formation of thiobarbituric acid relative substances (TBARS), relative electrophoretic mobility (REM), and fragmentation of apolipoprotein B (ApoB)-100.
Results : Calibration curves were acquired with r2 ¡Ã 0.9998. The contents of liquiritin, ferulic acid, baicalin, baicalein, glycyrrhizin, and wogonin in GMGHT were 1.784, 1.693, 37.899, 0.258, 1.869, and 0.034 mg/g, respectively. The GMGHT showed the radical scavenging activity in a dose-dependent manner. The concentration required for 50% reduction (RC50) against ABTS and DPPH radicals were 72.51 ¥ìg/mL and 128.49 ¥ìg/mL. Furthermore, GMGHT reduced the oxidation properties of LDL induced by CuSO4.
Conclusion : HPLC-PDA is considered as an available and convenient method for quality control and standardization of GMGH and GMGHT has potentials on anti-atherosclerosis by anti-oxidative effect and suppressive effect on LDL oxidation.
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KEYWORD
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Gumiganghwal-tang (Jiuweiqianghuo-tang), simultaneous analysis, ABTS, DPPH, LDL oxidation inhibition, anti-atherosclerosis
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